The use of metabolic engineering to produce fatty acid-derived biofuel and chemicals in Saccharomyces cerevisiae: a review
نویسندگان
چکیده
Production of fatty acid-derived biofuels and chemicals have garnered attention in recent years owing to their potential to replace petroleum and plant oil-derived products. Through the metabolic engineering of the fatty acid metabolism pathway, advanced fuels and chemicals such as free fatty acid, triacylglycerol, biodiesel, fatty alcohols, alkanes/alkene, R-3-hydroxybutyric acid, polyhydroxyalkanoates and flavonoids have been produced. The robustness, high tolerance to organic solvent, good reputation in industrial fermentations and excellent availability of genetic tools make the yeast Saccharomyces cerevisiae a suitable cell factory for fatty acid-derived biofuels and chemicals production. This review will describe the successful metabolic engineering strategies employed to produce the fatty acid-derived bio-products in S. cerevisiae, including the enhancement of precursors and co-factors supply, promotion of the enzyme expression and activity, elimination of competing pathways, and the improvement of strain tolerance.
منابع مشابه
Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals.
As the serious effects of global climate change become apparent and access to fossil fuels becomes more limited, metabolic engineers and synthetic biologists are looking towards greener sources for transportation fuels. In recent years, microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of trans...
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